DNA

Part:BBa_K4121006:Design

Designed by: Ziyu Li   Group: iGEM22_NEFU_China   (2022-10-04)


It is a promoter in Saccharomyces cerevisiae, and the expression intensity is relatively strong.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 451
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 451
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 451
    Illegal XhoI site found at 399
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 451
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 451
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 493


Design Notes

Before using this part, give priority to whether it has the enzyme cleavage site required, if so, consider site-directed mutagenesis.


Source

EPD (Eukaryotic Promoter Database) website

References